Journal: American Journal of Life Sciences PDF
Published: 12-Oct-15 Volume: 3 Issue: 5 Pages: 369-374
DOI: 10.11648/j.ajls.20150305.16 ISSN: 2328-5702
Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak , Mayank Gangwar, Snehasis Jana
Citation: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana. Antibiogram Typing of Biofield Treated Multidrug Resistant Strains of Staphylococcus Species. American Journal of Life Sciences. Vol. 3, No. 5, 2015, pp. 369-374. doi: 10.11648/j.ajls.20150305.16
Antimicrobial resistance is a global health issue in the developing countries. This study was carried out to evaluate the impact of Mr. Trivedi’s biofield energy treatment on multidrug resistant (MDR) clinical lab isolates (LSs) of Staphylococcus species viz. Staphylococcus haemolyticus (LS 18), Staphylococcus epidermidis (LS 21), and Staphylococcus aureus (LS 30). Each strain was divided into the two groups i.e.control and treated. The control and treated groups were analyzed for the antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical analysis and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment and compared with the control group. The sensitivity of erythromycin was improved from resistant to susceptible, while levofloxacin sensitivity was also improved from intermediate to susceptible in LS 21 isolate. The MIC results showed a decrease in the concentrations of ceftriaxone, erythromycin, imipenem, and levofloxacin antimicrobials in LS 21 as compared to the control. Linezolid and vancomycin also showed decrease in MIC as compared to the control in LS 30. Overall, 20.69% antimicrobials showed decrease in MIC value out of the tested twenty-nine after biofield treatment in Staphylococcus species. The biochemical study showed a 25% alteration in biochemical reactions as compared to the control. A significant change was reported in biotype numbers for all the three strains of MDR Staphylococcus species after biofield treatment as compared to the respective control group. On the basis of changed biotype number (306366) after biofield treatment in LS 18, the new organism was identified as Staphylococcus simulans with respect to the control species i.e. Staphylococcus haemolyticus (302302). The control group of S. epidermidis and S. aureus showed biotype number as 303064 and 757153 respectively. After biofield treatment, LS 21 and LS 30 isolates showed altered biotype number as 307064 and 317153 respectively. Overall, results conclude that biofield treatment could be used as complementary and alternative treatment strategy against multidrug resistant strains of Staphylococcus species with improved sensitivity and reduced MIC values of antimicrobial.
Overall results suggested the impact of biofield treatment on Staphylococcus species. Antimicrobial sensitivity results in improved sensitivity pattern of erythromycin, with eight-fold decrease in MIC value (i.e. >4 to ≤0.5 µg/mL), while, levofloxacin was also reported with improved sensitivity pattern with improved MIC value (4 to ≤2 µg/mL) in biofield treated S. epidermidis. Biofield treatment on Staphylococcus species showed altered effect on 25% tested biochemicals and biotype numbers. A significant change in biotype number (306366) on the basis of altered biochemical reactions as compared to control (302302). Altered biotype number results in identification of new organism as Staphylococcus simulans with respect to control species as S. haemolyticus in LS 18 after biofield treatment. Based on the results, Mr. Trivedi’s biofield energy treatment could be applied to improve the sensitivity pattern of antimicrobials, against multidrug resistance strains of Staphylococcus species.