Agronomic Characteristics, Growth Analysis, and Yield Response of Biofield Treated Mustard, Cowpea, Horse Gram, and Groundnuts

Journal: International Journal of Genetics and Genomics PDF

Published: 09-Nov-15 Volume: 3 Issue: 6

DOI:10.11648/j.ijgg.20150306.13 ISSN: 2376-7340 (Print) 2376-7359 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton , Dahryn Trivedi, Gopal Nayak , Mayank Gangwar, Snehasis Jana

Abstract

Mustard, cowpea, horse gram, and groundnuts are the seasonal pulse crops used as food and fodder in many regions of the world. In the present study, the impact of biofield energy treatment on mustard, cowpea, horse gram, and groundnuts were studied with respect to overall growth, yield, and its related yield attributes. Seeds of each crop was selected and divided in two groups, i.e. control and treated. The treated group of each seed crops was subjected to Mr. Trivedi biofield energy treatment, and were plotted in the separate fields. The plot with untreated seeds were provided with all the precautionary measures such as pesticides, fungicides and organic additives, while no such measures were taken in the plot with treated seeds. Both group of crops were further analyzed and compared for growth, yield, and yield attributes. Further, the effect of biofield treatment was also evaluated on horse gram using Random Amplified Polymorphic DNA (RAPD) analysis in order to determine their epidemiological relatedness and genetic characteristics. The results suggest that the percentage increase in yield was maximum in mustard (500%), followed by horse gram (105%), cow pea (52%), and groundnut (44%) as compared with their control. However, improved plant height, overall growth, yield of seeds, plants were free from any diseases and pest were observed in treated group as compared with its respective control. RAPD analysis using eight primers results in polymorphism and the percentage of true polymorphism observed between control and treated samples of horse gram seed sample with an average value of 53%. The overall results suggested that Mr. Trivedi’s biofield energy treatment has a significant impact on mustard, cowpea, horse gram, and groundnuts, which might be used as a better alternative approach to increase the yield of crop as compared with the synthetic chemicals.

Effect of Biofield Treatment on Physical, Thermal, and Spectral Properties of SFRE 199-1 Mammalian Cell Culture Medium

Journal: Advances in Biochemistry PDF

Published: 09-Nov-15 Volume: 3 Issue: 6

DOI:10.11648/j.ab.20150306.13 ISSN: 2329-0870 (Print) 2329-0862 (Online)

Authors: Mahendra Kumar Trivedi , Alice Branton, Dahryn Trivedi, Gopal Nayak , Khemraj Bairwa, Snehasis Jana

Abstract

SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Subsequently,the control and treated samples were analyzed using various analytical techniques. The CHNO analysis showed about 2.16, 4.87, and 5.89% decrease in percent contents of carbon, hydrogen, and oxygen, respectively; while 9.49% increase in nitrogen contents of treated sample as compared to the control. X-ray diffraction (XRD) analysis showed 7.23% decrease in crystallite size of treated sample as compared to the control. The thermogravimetric analysis (TGA) analysis showed the increase in onset temperature of thermal degradation by 19.61% in treated sample with respect to the control. The control sample showed the 48.63% weight loss during the thermal degradation temperature (Tmax) while the treated sample showed only 13.62% weight loss during the Tmax. The differential scanning calorimetry (DSC) analysis showed the 62.58% increase in the latent heat of fusion of treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated SFRE-M showed the alteration in the wavenumber of C-O, C-N and C-H vibrations in the treated sample as compared to the control. Altogether, the XRD, TGA-DTG, DSC, and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal and spectral properties of SFRE-M. The treated SFRE-M was more thermal stable than the control SFRE-M and can be used as the better culture media for mammalian cell culture.

Biochemical Differentiation and Molecular Characterization Of Biofield Treated Vibrio parahaemolyticus

Journal: American Journal of Clinical and Experimental Medicine PDF

Published: 09-Nov-15 Volume: 3 Issue: 5

DOI:10.11648/j.ajcem.20150305.21 ISSN: 2330-8125 (Print) 2330-8133 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on [ATCC-17802] strain of V. parahaemolyticus for its biochemical characteristics, biotype and 16S rDNA analysis. The lyophilized strain of V. parahaemolyticus was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, whereas, Gr. IIB was stored and analyzed on day 142 (Study I). After retreatment of Gr. IIB on day 142 (Study II), the sample was divided into three separate tubes. The tubes first, second and third were analyzed on day 5, 10, and 15, respectively. The biochemical reaction and biotyping were performed using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of V. parahaemolyticus with other bacterial species after the treatment. The results of biochemical reactions were altered 24.24%, out of thirty-three in the treated groups with respect to the control. Moreover, negative (-) reaction of urea was changed to positive (+) in the revived treated Gr. IIB, Study II on day 15 as compared to the control. Besides, biotype number was substantially changed in all the treated groups as compared to the control. However, change in organisms were reported in Gr. IIA on day 10 and in Gr. IIB; Study II on day 5 as Shewanella putrefaciens and Moraxella/Psychrobacter spp., respectively with respect to the control i.e. Vibrio sp. SF. 16S rDNA analysis showed that the identified sample in this experiment was V. parahaemolyticus after biofield treatment, and the nearest homolog genus-species was observed as Vibrio natriegens with 98% gene identity. The results envisaged that the biofield energy treatment showed an alteration in biochemical reaction pattern and biotype number on the strain of V. parahaemolyticus.

Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone

Journal: Advances in Bioscience and Bioengineering PDF

Published: 21-Dec-15 Volume: 3 Issue: 6

DOI:10.11648/j.abb.20150306.12 ISSN: 2330-4154 (Print) 2330-4162 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton , Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Abstract

Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Subsequently, both the samples were assessed using numerous analytical techniques. The X-ray diffractograms (XRD) showed the halo patterns of XRD peaks in both the samples. The particle size analysis exhibited about 4.70% and 17.58% increase in the d50 (average particle size) and d99 (particle size below which 99% particles are present), respectively of treated bio peptone as compared to the control. The surface area analysis revealed the 253.95% increase in the specific surface area of treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis showed the 29.59% increase in the melting temperature of treated bio peptone sample as compared to the control. Thermogravimetric analysis (TGA) showed the increase in onset of degradation temperature by 3.31% in the treated sample with respect to the control. The Fourier transform infrared (FT-IR) study revealed the changes in the wavenumber of functional groups such as O-H stretching from 3066 cm-1 to 3060 cm-1; C-H stretching from 2980, 2893, and 2817 cm-1 to2970, 2881, and 2835 cm-1, respectively; N-H bending from 1589 cm-1 to 1596 cm-1; C=C stretching from 1533 cm-1 to 1525 cm-1; and P=O stretching from 1070 cm-1 to 1078 cm-1 in treated sample as compared to the control. The UV-vis spectroscopy showed the similar patterns of absorbance maxima (?max) i.e. at 259 nm and 257 nm in both the control and treated samples, respectively. Overall, the analytical results suggested that Mr. Trivedi’s biofield energy treatment has substantial effect on physicochemical and spectral properties of bio peptone. Owing to this, the treated bio peptone might be more effective as culture medium than the corresponding control.

Physical, Spectroscopic and Thermal Characterization of Biofield Treated Fish Peptone

Journal: European Journal of Biophysics PDF

Published: 21-Dec-15 Volume: 3 Issue: 6

DOI:10.11648/j.ejb.20150306.12 ISSN: 2329-1745 (Print) 2329-1737 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

Abstract

The by-products of industrially processed fish are enzymatically converted into fish protein isolates and hydrolysates having a wide biological activity and nutritional properties. However, the heat processing may cause their thermal denaturation thereby causing the conformational changes in them. The present study utilized the strategy of biofield energy treatment and analysed its impact on various properties of the fish peptone as compared to the untreated (control) sample. The fish peptone sample was divided into two parts; one part was subjected to Mr. Trivedi’s biofield treatment, coded as the treated sample and another part was coded as the control. The impact of biofield treatment was analysed through various analytical techniques and results were compared with the control sample. The particle size data revealed 4.61% increase in the average particle size (d50) along with 2.66% reduction in the surface area of the treated sample as compared to the control. The X-ray diffraction studies revealed the amorphous nature of the fish peptone sample; however no alteration was found in the diffractogram of the treated sample with respect to the control. The Fourier transform infrared studies showed the alterations in the frequency of peaks corresponding to N-H, C-H, C=O, C-N, and C-OH, functional groups in the treated sample as compared to the control. The differential scanning calorimetry data revealed the increase in transition enthalpy (?H) from -71.14 J/g (control) to -105.32 J/g in the treated sample. The thermal gravimetric analysis data showed the increase in maximum thermal degradation temperature (Tmax) from 213.31°C (control) to 221.38°C along with a reduction in the percent weight loss of the treated sample during the thermal degradation event. These data revealed the increase in thermal stability of the treated fish peptone and suggested that the biofield energy treatment may be used to improve the thermal stability of the heat sensitive compounds.

Evaluation of Vegetative Growth Parameters in Biofield Treated Bottle Gourd (Lagenaria siceraria) and Okra (Abelmoschus esculentus)

Journal: International Journal of Nutrition and Food Sciences PDF

Published: 21-Dec-15 Volume: 4 Issue: 6

DOI:10.11648/j.ijnfs.20150406.24 ISSN: 2327-2694 (Print) 2327-2716 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

The objective of the study was to assess the growth contributing characters of biofield treated bottle gourd (Lagenaria siceraria) and okra (Abelmoschus esculentus) seeds. The seeds of both crops were divided into two groups, one was kept aside and denoted as untreated, while the other group was subjected biofield energy treatment. The variabilities in growth contributing parameters were studied and compared with their control. Further the level of glutathione (GSH) in okra leaves, along with DNA fingerprinting in bottle gourd were analyzed using RAPD method. After germination, the plants of bottle gourd were reported to be strong and erect with better canopy as compared with the control. The vegetative growth of okra plants after biofield energy treatment was found to be stout with small canopy, strong steam, and more fruits per nodes, that contributed high yield as compared with the control. However, endogenous level of GSH in the leaves of okra was increased by 47.65% as compared to the untreated group, which may suggest an improved immunity of okra crops. Besides, the DNA fingerprinting data, showed polymorphism (42%) between treated and untreated samples of bottle gourd. The overall results suggest that the biofield energy treatment on bottle gourd and okra seeds, results an improved overall growth of plant and yield, which may enhance flowering and fruiting per plant. Study results conclude that the biofield energy treatment could be an alternate method to improve the crop yield in agricultural science.

Morphological Characterization, Quality, Yield and DNA Fingerprinting of Biofield Energy Treated Alphonso Mango (Mangifera indica L.)

Journal: Journal of Food and Nutrition Sciences PDF

Published: 22-Dec-15 Volume: 3 Issue: 6

DOI:10.11648/j.jfns.20150306.18 ISSN: 2330-7285 (Print) 2330-7293 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

Alphonso is the most delicious variety of mango (Mangifera indica L.) known for its excellent texture, taste, and richness with vitamins and minerals. The present study was attempted to evaluate the impact of Mr. Trivedi’s biofield energy treatment on morphological characteristics, quality, yield and molecular assessment of mango. A plot of 16 acres lands used for this study with already grown mango trees. This plot was divided into two parts. One part was considered as control, while another part was subjected to Mr. Trivedi’s biofield energy treatment without physically touching and referred as treated. The treated mango trees showed new straight leaves, without any distortion and infection, whereas the control trees showed very few, distorted, infected, and curly leaves. Moreover, the flowering pattern of control trees did not alter; it was on average 8 to 10 inches with more male flowers. However, the flowering pattern of treated trees was completely transformed into compact one being 4 to 5 inches in length and having more female flowers. Additionally, the weight of matured ripened mango was found on an average 275 gm, medium sized with 50% lesser pulp in the control fruits, while the fruits of biofield energy treated trees showed on average weight of 400 gm, large sized and having 75% higher pulp as compared to the control. Apart from morphology, the quality and nutritional components of mango fruits such as acidity content was increased by 65.63% in the treated sample. Vitamin C content in the treated Alphonso mango pulp was 43.75% higher than the pulp obtained from the control mango farm. The spongy tissue content in pulp of the matured ripened mangoes was decreased by 100% for two consecutive years as compared to the control. Moreover, the yield of flowers and fruits in the treated trees were increased about 95.45 and 47.37%, respectively as compared to the control. Besides, the DNA fingerprinting data using RAPD revealed that the treated sample did not show any true polymorphism as compared to the control. The overall results envisaged that the biofield energy treatment on the mango trees showed a significant improvement in the morphology, quality and overall productivity along with 100% reduction in the spongy tissue disorder. In conclusion, the biofield energy treatment could be used as an alternative way to increase the production of quality mangoes.

Comparative Physicochemical Evaluation of Biofield Treated Phosphate Buffer Saline and Hanks Balanced Salt Medium

Journal: American Journal of BioScience PDF

Published: 21-Dec-15 Volume: 3 Issue: 6

DOI:10.11648/j.ajbio.20150306.20 ISSN: 2330-0159 (Print) 2330-0167 (online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

Abstract

Phosphate buffer saline (PBS) has numerous biological and pharmaceutical applications. Hank buffer salt (HBS) has been used as a medium for tissue culture applications. This research study was aimed to investigate the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of the PBS and HBS. The study was executed in two group’s i.e. control and treated. The control group was kept aside as control and treated group had received the biofield energy treatment. The control and treated samples were further characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and Fourier transform infrared (FT-IR) spectroscopy. The XRD analysis indicated the increase in crystallite size by 5.20% in treated PBS as compared to the control. Similarly, the treated HBS also showed increase in crystallite size by 3.20% with respect to the control. Additionally, the treated PBS showed an increase in Bragg’s angle (2?) as compared to the control sample. However, a decrease in Bragg’s angle of XRD peaks of the treated sample was noticed in the treated HBS. The DSC analysis of the control PBS showed melting temperature at 224.84°C; however melting temperature was not observed in the treated sample. However, DSC analysis of the treated HBS showed an increase in melting temperature (152.83°C) in comparison with the control (150.60°C). Additionally, the latent heat of fusion of the treated HBS was increased substantially by 108.83% as compared to the control. The TGA thermogram of the treated PBS showed an increase in onset of thermal degradation (212°C) as compared to the control (199°C). Whereas, the treated HBS showed less weight loss comparing with the control sample. This indicated the increase in thermal stability of the both the treated PBS and HBS samples. The FT-IR spectroscopic analysis of treated PBS showed alterations in the frequency of the functional groups such as O-H, C-H, P=O, O=P-OH, and P-OH as compared to the control. Additionally, the FT-IR spectrum of the treated HBS showed increase in frequency of calcium chloride phase (1444?1448 cm-1) as compared to the control sample. Altogether, it was observed that biofield energy treatment had caused physical, thermal and spectral changes in the treated samples as compared to the control. It is assumed that biofield energy treated PBS and HBS could be a good prospect for biological and tissue culture applications.

Physicochemical and Spectroscopic Characterization of Yeast Extract Powder After the Biofield Energy Treatment

Journal: American Journal of Life Sciences PDF

Published: 21-Dec-15 Volume: 3 Issue: 6

DOI:10.11648/j.ajls.20150306.12 ISSN: 2328-5702 (Print) 2328-5737 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Abstract

Yeast extract powder (YE powder) is particularly used in culture media for the cultivation of microorganisms found in milk or other dairy products. The present study was intended to explore the influence of biofield energy treatment on the physicochemical and spectral properties of YE powder. The study was accomplished in two groups; first group was remained as control, while another was subjected to Mr. Trivedi’s biofield energy treatment and termed as the treated group. Afterward, both the samples were evaluated using several analytical techniques. The X-ray diffractometry (XRD) study showed the halo patterns of XRD peaks in both the samples. This indicated the amorphous nature of the samples. The particle size study revealed the 4.77% and 26.28% increase d50 (in the average particle size) and d99 (particle size below that 99% particles are present), respectively of treated YE powder with respect to the control. The surface area analysis showed the 14.06% increase in the specific surface area of treated sample with respect to the control. The differential scanning calorimetry (DSC) analysis exhibited the 41.64% increase in the melting temperature of treated YE powder sample as compared to the control. The TGA/DTG analysis exhibited the increase in Tonset (onset temperature of thermal degradation) by 7.51% and 12.45% in first and second step of thermal degradation, respectively in the treated sample as compared to the control. Furthermore, the Tmax (maximum thermal degradation temperature) was increased by 4.16% and 24.79% in first and second step of thermal degradation, respectively in the treated sample with respect to the control. The Fourier transform infrared (FT-IR) study revealed the changes in the wavenumber of functional groups such as C-H (stretching) from 2895?2883 cm-1 and 2815?2831 cm-1, respectively; C-N from 1230?1242 cm-1; and C-O stretching from 1062-1147 cm-1?1072-1149 cm-1 of treated YE powder sample as compared to the control. The UV-vis spectroscopy showed the similar patterns of absorbance maxima (?max) in both the control and treated samples. Therefore, the analytical results suggested the considerable impact of Mr. Trivedi’s biofield energy treatment on physicochemical and spectral properties of YE powder. The increase in Tonset and Tmax after the biofield treatment suggests that the treated YE powder might be more effective in culture medium than the control YE powder.

Impact of Biofield Energy Treatment on Soil Fertility

Journal: Earth Sciences PDF

Published: 21-Dec-15 Volume: 4 Issue: 6

DOI:10.11648/j.earth.20150406.19 ISSN: 2328-5974 (Print) 2328-5982 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

Measurement of soil components such as microbial population, minerals and obviously the content of organic carbon play the important roles for the productivity of crops and plants. The present study was attempted to evaluate the impact of Mr. Trivedi’s biofield energy treatment on soil for its physical (electrical conductivity), chemical (minerals) and microbial flora (bacteria and fungi). A plot of lands was assigned for this study with some already grown plants. This plot was divided into two parts. One part was considered as control, while another part was subjected to Mr. Trivedi’s biofield energy treatment without physically touching and referred as treated. In the treated soil the total bacterial and fungal counts were increased by 546 and 617%, respectively as compared to the untreated soil. Additionally, the conductivity of soil of the treated plot was increased by 79% as compared to the soil of control plot. Apart from microbes, the content of various minerals were also changed in the biofield energy treated soil. The calcium carbonate content showed 2909 ppm in the control, while in the treated soil it was increased to 3943 ppm i.e. 36% increased. Various other minerals such as nitrogen and potassium were increased by 12% and 7%, respectively as compared to the control. Besides, the level of some minerals such as potassium, iron, and chloride were decreased by 9%, 23%, and 41%, respectively as compared to the control. Apart from chemical constituents of soil, the content of organic carbon was also reduced by 8% in the treated soil as compared to the control soil. The overall results envisaged that the biofield energy treatment on the soil showed a significant improvement in the physical, chemical, and microbial functions of soil component. Thus, improved the conductance, supportive microbes, minerals and overall productivity of crops. In conclusion, the biofield energy treatment could be used as an alternative way to increase the yield of quality crops by increasing soil fertility.

Morphological and Molecular Analysis Using RAPD in Biofield Treated Sponge and Bitter Gourd

Journal: American Journal Of Agriculture And Forestry PDF

Published: 21-Dec-15 Volume: 3 Issue: 6

DOI:10.11648/j.ajaf.20150306.14 ISSN: 2330-8581 (Print) 2330-8591 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

Plants are known to have sense and can respond to touch, electric and magnetic field. The present study was designed on the sponge gourd (Luffa cylindrica) and bitter gourd (Momordica charantia) seeds with respect to biofield energy treatment. The seeds of each crop were divided into two groups, one was kept control, while the other group was subjected to Mr. Trivedi’ biofield energy treatment. The variabilities in growth contributing parameters were studied and compared with their control. To study the genetic variability after biofield energy treatment, both the seeds were analyzed for DNA fingerprinting using RAPD method. After germination, the plants of sponge gourd were reported to have uniform colored leaves and strong stem. The leaves and fruits of sponge gourd showed no infection, with anti-gravity properties during early stage of fruiting as compared with the control group. Similarly, treated bitter gourd showed uniform color of leaves, strong stem, with disease free fruits in biofield treated seeds as compared with the control. The true polymorphism (%) observed between control and treated samples of sponge and bottle gourd seed sample was an average value of 7.8% and 66% respectively. In conclusion, Mr. Trivedi’s biofield energy treatment has the ability to alter the plant growth rate that may by interacting with plant genome, which resulted in high yield of crops.

Physicochemical Evaluation of Biofield Treated Peptone And Malmgren Modified Terrestrial Orchid Medium

Journal: American Journal of Bioscience and Bioengineering PDF

Published: 21-Dec-15 Volume: 3 Issue: 6

DOI:10.11648/j.bio.20150306.15 ISSN: 2328-5885 (Print) 2328-5893 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

Abstract

Peptone and Malmgren modified terrestrial orchid (MMTO) has been used as a growth medium for tissue culture applications. This research study was conducted to explore the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of peptone and MMTO. The study was performed in two groups i.e. control and treated. The control group was kept aside as untreated, and the treated group was received the biofield energy treatment. The control and treated samples were further subjected to characterization by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer and surface area analyzer. The XRD analysis revealed the amorphous nature of the control and treated peptone samples. The DSC analysis showed an increase in thermal denaturation temperature of the treated peptone (196.22°C) as compared to the control sample (141.20°C). Additionally, the exothermic peak of treated sample (280°C) was increased as compared to the control (270°C). The DSC of control and treated MMTO showed the absence of the melting temperature in their respective DSC thermograms. The TGA analysis of the treated peptone showed an increase in onset of thermal degradation (172°C) with respect to the control (170°C). Nevertheless, the TGA thermogram of the treated MMTO (293.96°C) showed an increase in maximum thermal degradation temperature (Tmax) as compared with the control (281.41°C). It indicated the good thermal stability of the treated peptone and MMTO samples. The FT-IR result of the treated peptone showed an upward shift in C-H (2817?2833 cm-1), and amide I (1635?1641 cm-1), stretching in the treated sample with respect to the control sample. Whereas, the FT-IR spectrum of the treated MMTO showed an increase in the frequency of the C-H (2817?2833 cm-1) and amide I (1596?1606 cm-1) bands as compared to the control. Particle size analysis of the treated peptone showed an increase in d50 (average particle size) and d99 (size exhibited by 99% of particles) by 9.3 and 41.4%, respectively with respect to the control. Surface area analysis showed increase in surface area by 4.3% in the treated peptone. Altogether, the results corroborated that the biofield energy treatment had altered the physical, thermal and spectral properties of peptone and MMTO. It is assumed that biofield treated peptone and MMTO could be utilized as potential candidates for cell culture applications.

Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Murashige and Skoog Plant Cell Culture Media

Journal: Cell Biology PDF

Published: 22-Dec-15 Volume: 3 Issue: 4

DOI:10.11648/j.cb.20150304.11 ISSN: 2330-0175 (Print) 2330-0183 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Abstract

The Murashige and Skoog medium (MS media) is a chemically defined and widely used as a growth medium for plant tissue culture techniques. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MS media. The study was performed in two groups; one was kept as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Afterward, both the control and treated samples were analyzed using various analytical techniques. The X-ray diffraction (XRD) analysis showed 19.92% decrease in the crystallite size of treated sample with respect to the control. The thermogravimetric analysis (TGA) showed the increase in onset temperature of thermal degradation (Tonset) by 9.41% and 10.69% in first and second steps of thermal degradation, respectively after the biofield energy treatment as compared to the control. Likewise, Tmax (maximum thermal degradation temperature) was increased by 17.43% and 28.61% correspondingly in the first and second step of thermal degradation in the treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis indicated the 143.51% increase in the latent heat of fusion of the treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated MS media showed the alteration in the frequency such as 3165?3130 cm-1 (aromatic C-H stretching); 2813?2775 cm-1 (aliphatic C-H stretching); 1145?1137 cm-1 (C-N stretching), 995?1001 cm-1 (S=O stretching), etc. in the treated sample with respect to the control. The UV spectra of control and treated MS media showed the similar absorbance maxima (?max) i.e. at 201 and 198 nm, respectively. The XRD, TGA-DTG, DSC, and FT-IR results suggested that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal, and spectral properties of the MS media. As a result, the treated MS media could be more stable than the control, and might be used as better media in the plant tissue culture technique.

Evaluation of Plant Growth, Yield and Yield Attributes of Biofield Energy Treated Mustard (Brassica juncea) and Chick Pea (Cicer arietinum) Seeds

Journal: Agriculture, Forestry and Fisheries PDF

Published: 22-Dec-15 Volume: 4 Issue: 6

DOI:10.11648/j.aff.20150406.19 ISSN: 2328-563X (Print) 2328-5648 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Abstract

The present study was carried out to evaluate the effect of Mr. Trivedi’s biofield energy treatment on mustard (Brassica juncea) and chick pea (Cicer arietinum) for their growth, yield, and yield attributes. Both the samples were divided into two groups. One group was remained as untreated and coded as control, while the other group (both seed and plot) was subjected to Mr. Trivedi’s biofield energy treatment and referred as the treated. The result showed the plant height of mustard and chick pea was increased by 13.2 and 97.41%, respectively in the treated samples as compared to the control. Additionally, primary branching of mustard and chick pea was improved by 7.4 and 19.84%, respectively in the treated sample as compared to the control. The control mustard and chick pea crops showed high rate of infection by pests and diseases, while treated crops were free from any infection of pests and disease. The yield attributing characters of mustard showed, lucidly higher numbers of siliquae on main shoot, siliquae/plant and siliquae length were observed in the treated seeds and plot as compared with the control. Moreover, similar results were observed in the yield attributing parameters of chick pea viz. pods/plant, grains/pod as well as test weight of 1000 grains. The seed and stover yield of mustard in treated plots were increased by 61.5% and 25.4%, respectively with respect to the control. However, grain/seed yield of mustard crop after biofield energy treatment was increased by 500% in terms of kg per meter square as compared to the control. Besides, grain/seed yield of chick pea crop after biofield energy treatment was increased by 500% in terms of kg per meter square. The harvest index of biofield treated mustard was increased by 21.83%, while it was slight increased in case of chick pea. In conclusion, the biofield energy treatment could be used on both the seeds and plots of mustard and chick pea as an alternative way to increase the production and yield.

Effect of Biofield Energy Treatment on Chlorophyll Content, Pathological Study, and Molecular Analysis of Cashew Plant (Anacardium occidentale L.)

Journal: Journal of Plant Sciences PDF

Published: 21-Dec-15 Volume: 3 Issue: 6

DOI:10.11648/j.jps.20150306.21 ISSN: 2331-0723 (Print) 2331-0731 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

In the world scenario, India occupies a premier position contributing to about 43 per cent production of the cashew nut (Anacardium occidentale L.) along with export and processing. The aim is to study the impact of biofield energy treatment on selected farms for cashew farming. The control and biofield treated farms were divided as control and treated farms, and Mr. Trivedi provided the biofield energy treatment to the treated farms. Further, the plants and fruits were analyzed for overall growth of plants, chlorophyll content, productivity, pathological study, and shelf life using UN specifications for International Trade, biophoton emission study, and DNA fingerprinting using RAPD method. No chemicals, fertilizers, were used on the treated plot, although regular practices were followed on control farms such as fertilizers, pesticides and fungicides due to the high incidence of disease and the requirement of nutritional supplements in the region. The analysis showed that biofield treated farm plants have thicker and stronger branches with more secondary and tertiary branches, flowering pattern, and canopy of plants was improved than trees of the same variety along with height of the plants, as compared with the control. The results showed that chlorophyll a and b content in biofield treated lands plants were increased by approximately 30% and 93% respectively, while total chlorophyll content by 45% as compared with the control. The pathological examination showed the presence of fungi namely Colletotrichum gloeosporioides and Botryodiplodia theobromae in control, which were absent in treated plants. Biophoton study suggested that the cashew fruits were bigger in size with high density, strength, and vitality as compared with the control. The shelf life analysis reflected that the biofield treated cashews showed sweet taste, and can be stored for longer duration due to less moisture, and altered minerals content, such as high iodine, and low p-anisidine level. RAPD analysis showed a high level of polymorphism among control and treated samples, while level of true polymorphism among V4 variety of cashew was ranges from 0 to 100%, and in V7 variety, it ranged from 25 to 91% using different set of RAPD primers. Overall, study results suggest that Mr. Trivedi’s biofield energy treatment on land planted with cashew could be an alternative approach to improve the overall growth of plant, and fruit yield.

Assessment of Antibiogram of Biofield Energy Treated Serratia marcescens

Journal: European Journal of Preventive Medicine PDF

Published: 22-Dec-15 Volume: 3 Issue: 6

DOI:10.11648/j.ejpm.20150306.18 ISSN: 2330-8222 (Print) 2330-8230 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Abstract

Serratia marcescens (S. marcescens) has become an important nosocomial pathogens and increased resistant isolates were reported. The current study evaluates the impact of an alternate energy medicine i.e. Mr. Trivedi’s biofield energy treatment on S. marcescens for changes in sensitivity pattern of antimicrobial, biochemical characteristics, and biotype number. S. marcescens cells were procured from MicroBioLogics Inc., USA in sealed pack bearing the American Type Culture Collection (ATCC 13880) number and divided into two groups, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two sub-groups, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes as first, second and third tube, which were analyzed on day 5, 10 and 15 respectively. All experimental parameters were studied using the automated MicroScan Walk-Away® system. Antimicrobial susceptibility results showed that 42.85% of tested antimicrobials results in altered sensitivity pattern, while decreased minimum inhibitory concentration values in 40.62% tested antimicrobials as compared to the control after biofield treatment on S. marcescens. The biochemical study showed that 12 out of 33 tested biochemicals (36.36%) were reported for alteration of biochemical reactions pattern as compared to the control. Biotype study showed an alteration in biotype number in all the experimental treated groups as compared to the control. These results suggested that biofield energy treatment has a significant impact on S. marcescens. Overall, it is expected that Mr. Trivedi’s biofield energy treatment as an integrative medicine could be better therapy approach in near future.

Molecular Analysis of Biofield Treated Eggplant and Watermelon Crops

Journal: Advances in Crop Science and Technology PDF

Published: 31-Jan-16 Volume: 4 Issue: 1

DOI:10.4172/2329-8863.1000208 ISSN: 2329-8863

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar and Snehasis Jana *

Abstract

Eggplant and watermelon, as one of the important vegetative crops have grown worldwide. The aim of the present study was to analyze the overall growth of the two inbreed crops varieties after the biofield energy treatment. The plots were selected for the study, and divided into two parts, control and treated. The control plots were left as untreated, while the treated plots were exposed with Mr. Trivedi’s biofield energy treatment. Both the crops were cultivated in different fields and were analyzed for the growth contributing parameters as compared with their respective control. To study the genetic variability in both plants after biofield energy treatment, DNA fingerprinting was performed using RAPD method. The eggplants were reported to have uniform colored, glossy, and greener leaves, which are bigger in size. The canopy of the eggplant was larger with early fruiting, while the fruits have uniform shape and the texture as compared with the control. However, the watermelon plants after the biofield treatment showed higher survival rate, with larger canopy, bright and dark green leaves compared with the untreated plants. The percentage of true polymorphism observed between control and treated samples of eggplant and watermelon seed samples were an average value of 18% and 17%, respectively. Overall, the data suggest that Mr. Trivedi’s biofield energy treatment has the ability to alter the plant growth rate, and can be utilized in better way as compared with the existing agricultural crop improvement techniques to improve the overall crop yield.

Antimicrobial Susceptibility Pattern and Biochemical Characteristics of Staphylococcus aureus: Impact of Bio field Treatment

Journal: Microbial & Biochemical Technology PDF

Published: 30-Jul-15 Volume: 7 Issue: 4

DOI:10.4172/1948-5948.1000215 ISSN: 1948-5948

Authors: Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Sambhu Charan Mondal and Snehasis Jana*

Abstract

Study background: Staphylococci are widespread in nature, mainly found on the skin and mucous membranes. Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of biofield treatment on antimicrobial susceptibility pattern and biochemical characteristics of S. aureus (ATCC 25923).

Methods: S. aureus cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 25923) number and stored according to the recommended storage protocols until needed for experiments. Revived and lyophilized state of ATCC strains of S. aureus were selected for the study. Both revived (Group; Gr. II) and lyophilized (Gr. III) strain of S. aureus were subjected to Mr. Trivedi’s biofield treatment. Revived treated cells were assessed on day 5 and day 10 while lyophilized treated cells on day 10 only. After biofield treatment both treated cells were analysed for its antimicrobial sensitivity, minimum inhibitory concentration value, biochemical reactions and biotype number with respect to control (Gr. I).

Results: The antimicrobial susceptibility and minimum inhibitory concentration of S. aureus showed significant (86.67%) alteration in lyophilized cells while no alteration was found in revived treated cells as compared to control. It was observed that overall 37.93% (eleven out of twenty nine) biochemical reactions were altered in the treated groups with respect to control. Moreover, biotype numbers were substantially changed in revived treated cells, Gr. II (303137, Staphylococcus capitis subsp. ureolyticus) on day 5 and in lyophilized treated cells, Gr. III (767177, S. cohnii subsp. urealyticum) on day 10 as compared to control (307016, S. aureus).

Conclusion: The result suggested that biofield treatment has significant impact on S. aureus in lyophilized treated cells with respect to antimicrobial susceptibility, MIC values and biochemical reactions pattern. Apart from these, biotype numbers with new species were observed in revived treated group on day 5 as Staphylococcus capitis subsp. ureolyticus and in lyophilized cells as Staphylococcus cohnii subsp. urealyticum with respect to control, i.e., S. aureus.

Impact of Biofield Treatment on Growth and Yield of Lettuce and Tomato

Journal: Australian Journal of Basic and Applied Sciences PDF

Published: 2012 Volume: 6 Issue: 10

DOI:Not Available ISSN: 1991-8178

Authors: Vishal Shinde, Frank Sances, Shrikant Patil and Amy Spence

Abstract

Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca sativa var. capitata and Lycopersiconesculentum var. Roma) in commercial plantings with and without fertilizers and pesticides, in relation to yield, quality, and pest inhibition. Treated lettuce plants with fertilizer and pesticide applications were more vigorous, exhibited less incidence of soil-borne fungal wilt, and subsequent yield was statistically greater 43% compared to untreated plants. Treated plants with no fertilizer or pesticide applications in the field behaved similarly to untreated plants that received routine fertilizer and pest control inputs. Similarly, fertilizer applied and fertilizer non-applied treated tomato plants exhibited a 25% and 31% increase in total observable yields respectively. Treated tomato and lettuce plants also measured higher in total leaf tissue chlorophyll content. The combination of biofield treatment along with administration of chemical additives demonstrated the best results with statistically increased yields and higher pest resistance in both test cropping systems. The specific mechanisms that lead to these preliminary results have yet to be determined.

Biofield and Fungicide Seed Treatment Influences on Soybean Productivity, Seed Quality and Weed Community

Journal: Agricultural Journal PDF

Published: 2013 Volume: 8 Issue: 3

DOI:10.3923/aj.2013.138.143 ISSN: 1816-9155

Authors: A.W. Lenssen

Abstract

Soybean production in Iowa USA is among the most productive for raínfed regions in the world. Despite generally having excellent soils, growing season temperatures and rainfall, soybean yields are decreased by weed interference and inadequate available soil water at key stages of crop development. A field study was conducted at two locations in lowa in 2012 to determine if seed-applied fungicide or biofield treatments influenced weed community, soil volumetric water concentration and soybean yield and quality. Application of biofield treatment resulted in lower density of tall waterhemp density, greater soybean stand density at R8 stage and greater seed pod-1 compared to the absence of seed fungicide and biofield­ Soil volumetric water content varied by seed fungicide x biofield x date interaction but differences were not consistent among treatment combinations. Overall, seed fungicide and biofield treatments had similar effects on soybean productivity, however additional research is necessary to determine if biofield treatment is a suitable replacement for seed fungicide application.

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