Evaluation of the Physico-chemical, Thermal and Behavioral Properties of Ashwagandha Root Extract: Effects of Consciousness Energy Healing Treatment

Journal: International Journal of Pharmacy and Chemistry PDF

Published: 10-May-17 Volume: 3 Issue: 3

DOI:10.11648/j.ijpc.20170303.13 ISSN: Not Available

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

Withania somnifera (Ashwagandha) root extract has a broad range of pharmacological activities due to the presence of many biologically active metabolites. The current study was designed to evaluate the influence of The Trivedi Effect® – Consciousness Energy Healing Treatment (Biofield Energy Healing) on the physical, spectroscopic, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis exhibited that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were increased significantly by 8.69%, 7.32%, and 6.50%, respectively compared with the control sample. Similarly, the surface area of the treated sample was significantly decreased by 12.69% compared to the control sample. The FT-IR analysis indicated no significant alteration of the force constant for the functional groups in the treated sample compared to the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 207.5 and 205.2 nm, respectively. The TGA revealed the two major thermal degradation steps and the total weight loss was decreased by 0.86% in the treated sample compared with the control sample. Consequently, the maximum thermal degradation temperature at 273.53°C and 389.39°C for two broad peaks in treated sample was increased by 0.78% and 0.31%, respectively compared to the control the sample (271.40°C and 388.48°C). The DSC analysis indicated that the evaporation temperature was significantly increased by 10.95% in the Biofield Energy Treated sample with a 5.34% reduced the latent heat of vaporization compared with the control sample. The overall thermal analysis indicated that the treated sample was thermally more stable as compared to the control sample. The Trivedi Effect® – Energy of Consciousness Healing Treatment might lead to have better powder flowability and long-term storage stability compared with the control sample. Thus, the treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, ageing, and other chronic infections.

LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

Journal: Journal of Drug Design and Medicinal Chemistry PDF

Published: 18-Apr-17 Volume: 3 Issue: 2

DOI:10.11648/j.jddmc.20170302.11 ISSN: 2472-355X (Print) 2472-3576 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

Withania somnifera root extract which contains withanolides as major active constituent and it is used traditionally for the prevention and treatment of several diseases. The objective of the current study was to investigate the impact of The Trivedi Effect® – Consciousness Energy Healing Treatment on the structural properties of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while other part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time of the phytoconstituents remained same in the control and Biofield Energy Treated samples, whereas the peak area% i.e. the relative amount of the phytoconstituents at respective retention time was significantly altered. The peak area% at Rt of 5.6, 6.8, 6.9, 7.2, 7.9, 8.4, 8.5, 8.6, and 9.2 minutes of the treated sample were increased significantly in the range of 1.46% to 253.06% compared to the control sample. In the contrary, the peak area% of the treated sample at Rt of 6.4 and 8.2 minutes were significantly decreased by 12.72% and 17.35%, respectively with respect to the control sample. A total of 16 withanolides such as withanoside IV, coagulin Q, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A, withanolide sulfoxide, withanolide B, etc. were proposed with their structure from the molecular mass at m/z 783, 621, 489, 473, 767, 471, 505, and 992 at retention times of 6.4, 6.8, 7.2, 7.9, 8.2, 8.4, and 9.1 minutes, respectively with the help of LC-MS, GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensities of the Biofield Energy Treated sample were significantly changed in the range of -81.36% to 1720.90% compared with the control sample at the same retention time. These findings suggest that The Trivedi Effect® – Consciousness Energy Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging, and other chronic infections.

Consciousness Energy Healing Treatment on Withania somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Journal: American Journal of Chemical Engineering PDF

Published: 9-May-17 Volume: 5 Issue: 2

DOI:10.11648/j.ajche.20170502.12 ISSN: 2330-8605 (Print) 2330-8613 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, James Jeffery Peoples, James Joseph Meuer, Johanne Dodon, John Lawrence Griffin, John Suzuki, Joseph Michael Foty, Judy Weber, Julia Grace McCammon, Karen Brynes Allen, Kathryn Regina Sweas, Lezley Jo-Anne Wright, Lisa A. Knoll, Madeline E. Michaels, Margaret Kweya Wahl, Mark E. Stutheit, Michelle Barnard, Muriel Mae Ranger, Paromvong Sinbandhit, V. J. Kris Elig, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Abstract

Withania somnifera (Ashwagandha) is useful as an herbal medicine and nutraceuticals for the prevention and treatment of anxiety, insomnia, tuberculosis, tumors, menstrual problems, etc. The objective of the current study was to evaluate the influence of The Trivedi Effect® – Consciousness Energy Healing Treatment on the physical, spectroscopic, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis demonstrated that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were decreased significantly by 18.88%, 8.60%, and 4.00%, respectively compared with the control sample. Similarly, the surface area of the treated sample was significantly increased by 102.63% compared to the control sample. FT-IR results suggest that there was a small impact of Biofield Energy Treatment on ashwagandha root extract at the atomic level to reduce the force constant of O-H (str.) bond. UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 207.4 and 207.7 nm, respectively. TGA revealed the three steps of thermal degradation steps and the total weight loss was decreased by 0.13% in the treated sample compared with the control sample. Consequently, the maximum thermal degradation temperature at 272.73°C and 393.14°C for two broad peaks in treated sample was increased by 1.13% and 0.85%, respectively compared to the control the sample (269.68°C and 389.83°C). DSC analysis indicated that the evaporation temperature and latent heat of vaporization was increased by 1.35% and 5.52%, respectively in the treated sample compared with the control sample. The current findings suggested that The Trivedi Effect® – Energy of Consciousness Healing Treatment might have the astounding capacity to enhance the solubility, absorption, dissolution, bioavailability and thermal stability of ashwagandha root extract in various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, sexual disorders, stress, cancer, diabetes, ageing, and other chronic infections.

Study of the Consciousness Energy Healing Treated Ashwagandha Root Extract by LC-MS, GC-MS, and NMR Spectroscopy

Journal: American Journal of Applied and Industrial Chemistry PDF

Published: 18-Apr-17 Volume: 3 Issue: 2

DOI:10.11648/j.ajaic.20170302.13 ISSN: Not Available

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, James Jeffery Peoples, James Joseph Meuer, Johanne Dodon, John Lawrence Griffin, John Suzuki, Joseph Michael Foty, Judy Weber, Julia Grace McCammon, Karen Brynes Allen, Kathryn Regina Sweas, Lezley Jo-Anne Wright, Lisa A. Knoll, Madeline E. Michaels, Margaret Kweya Wahl, Mark E. Stutheit, Michelle Barnard, Muriel Mae Ranger, Paromvong Sinbandhit, V. J. Kris Elig, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Abstract

Withania somnifera (ashwagandha) contains many biologically active constituents used for the prevention and treatment of various diseases. The aim of the current study was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. LC-MS data revealed that the retention time of the phytoconstituents remained same in the control and Biofield Energy Treated samples, whereas the peak area% i.e. the relative amount of the phytoconstituents was significantly altered. The peak area% at Rt of 5.1, 5.7, 6.4, 6.6, 6.9, 7.1, 7.4, 7.8, 7.9, 8.0, 8.2, 8.5, and 8.9 minutes of the treated sample were increased in the range of 0.92% to 14.67% compared to the control sample. On the contrary, the peak area% of the treated sample at Rt of 5.2, 5.3, 5.4, 5.6, 6.8, 8.1, 8.4, 8.6, 9.0, 9.1, and 9.2 minutes were decreased by 1.59% to 36.69% with respect to the control sample. A total of 14 withanolides such as sitoindoside IX, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at retention times of 6.9, 7.1, 7.9, 8.2, 8.5, and 9.3 minutes with the help of LC-MS, GC-MS and NMR data of both the control and Biofield Energy Treated samples. Consequently, the mass peak intensities of the treated sample were significantly changed in the range of -36.67% to 106% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.

Solid State Characterization of Withania somnifera (Ashwagandha) Root Extract After Treatment with Consciousness Energy Healing

Journal: Science Journal of Analytical Chemistry PDF

Published: 13-Apr-17 Volume: 5 Issue: 2

DOI:10.11648/j.sjac.20170502.11 ISSN: 2376-8405 (Print) 2376-8053 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

Ashwagandha root extract is useful as an herbal medicine and nutraceuticals for the prevention and treatment of various diseases. The aim of the current study was to evaluate the influence of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the physico-chemical, thermal and behavioral properties of ashwagandha root extract using powder X-ray diffraction (PXRD), particle size distribution analysis (PSD), Fourier transform infrared (FT-IR) spectrometry, UV-Vis spectroscopy, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment, and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis concluded that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were significantly decreased by 36.78%, 15.18%, and 5.06%, respectively compared with the control sample. Likewise, the surface area of the treated sample was significantly increased by 85.14% compared to the control sample. FT-IR results showed a small impact of Consciousness Energy Healing Treatment on the phytoconstituents of ashwagandha root extract to reduce the force constant of O-H (str.) bond. UV-vis analysis revealed that the wavelength for the maximum absorbance (λmax) of both the samples was at 206.4 in methanol. TGA revealed the three steps of thermal degradation and the total weight loss was decreased by 0.73% in the treated sample compared to the control sample. Consequently, the maximum thermal degradation temperature was found at 272.53°C and 393.35°C for two broad peaks in the treated sample was increased by 0.05% and 0.08%, respectively compared to the control the sample (272.67°C and 393.66°C). The DSC analysis indicated that the evaporation temperature and latent heat of vaporization were lowered significantly by 4.98% and 35.67%, respectively in the treated sample compared with the control sample. The current outcomes suggested that the Energy of Consciousness Healing Treatment might have the amazing capacity to enhance the solubility, dissolution, absorption, bioavailability and thermal stability of ashwagandha root extract in the various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the Biofield Energy Treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, sexual disorders, arthritis, stress, cancer, ageing, diabetes, and other chronic infections.

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Journal: European Journal of Biophysics PDF

Published: 8-May-17 Volume: 5 Issue: 2

DOI:10.11648/j.ejb.20170502.12 ISSN: 2329-1745 (Print) 2329-1737 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Abstract

Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.

A Systematic Study of the Biofield Energy Healing Treatment on Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate

Journal: International Journal of Bioorganic Chemistry PDF

Published: 1-May-17 Volume: 2 Issue: 3

DOI:10.11648/j.ijbc.20170203.19 ISSN: Not Available

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Abstract

Magnesium gluconate is a pharmaceutical/nutraceutical compound used as a source of magnesium ion. The recent study described the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment on magnesium gluconate for the variation in physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control without any Biofield Energy Treatment, while another part was treated with The Trivedi Effect®-Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The PXRD analysis exhibited that the crystallite size of the treated sample was remarkably altered from -70% to 130% compared with the control sample. The average crystallite size was significantly decreased by 23.74% in the treated sample compared to the control sample. Biofield Energy Healing Treatment significantly reduced the particle size of magnesium gluconate at d10, d50, and d90 values by 12.15%, 8.98% and 15.35%, respectively compared to the control sample. The surface area analysis showed that surface area of the treated sample was significantly increased by 11.76% compared with the control sample. The FT-IR and UV-vis analysis displayed that structure of the magnesium gluconate persisted identical in both the treated and control samples. The TGA analysis exhibited four steps thermal degradation in both samples and the total weight loss of the Biofield Energy Treated sample was reduced by 0.19% compared with the control sample. The melting temperature of the Biofield Energy Treated sample (171.25ºC) was slightly (0.16%) higher from the control sample (170.97ºC). The latent heat of fusion was significantly decreased by 7.76% in the treated sample compared to the control sample. The TGA and DSC analysis revealed that the thermal stability of the treated sample was enhanced compared with the control sample. The current study revealed that The Trivedi Effect®-Energy of Consciousness Healing Treatment might produce a new polymorphic form of magnesium gluconate, which could be more soluble and bioavailable along with improved thermal stability compared with the untreated compound. The Biofield Treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. Hence, The Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging, and other chronic infections.

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